کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496280 1116118 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination and pharmacokinetics of sixteen Angelicae dahurica coumarins in vivo by LC–ESI-MS/MS following oral delivery in rats
ترجمه فارسی عنوان
اندازه گیری همزمان و فارماکوکینتیک شانزده coumarins Angelicae dahurica در داخل بدن توسط LC-ESI-MS / MS پس از زایمان در رت
کلمات کلیدی
LC-ESI-MS / MS؛ طب سنتی چینی؛ Angelicae Dahuricae Radix؛ کومارین ها؛ PharmacokineticsLC-MS / MS، جرم کروماتوگرافی مایع؛ ADR، Angelicae dahuricae Radix؛ MRM، چند واکنش حالت؛ TCM، طب سنتی چینی؛ GABA، gamma-ami
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی

BackgroundThe roots of Angelica dahurica cv. Qibaizhi is frequently used in clinical practice as a traditional Chinese medicine. However, a comprehensive study of the pharmacokinetics of this medicine has not been carried out.MethodA sensitive and specific liquid chromatographic–tandem mass (LC–MS/MS) spectrometric method was established to investigate pharmacokinetics of sixteen coumarins of Angelicae dahuricae Radix (ADR) in rat plasma, including xanthotoxol (1), oxypeucedanin hydrate (2), 5-hydroxy-8-methoxypsoralen (3), (-)-marmesin (4), byakangelicin (5), columbianetin (6), psoralen (7), xanthotoxin (8), neobyakangelicol (9), isoimpinellin (10), bergapten (11), heraclenin (12), oxypeucedanin ethanolate (13), imperatorin (14), phellopterin (15), isoimperatorin (16). Detection was performed on a triple quadrupole mass spectrometer in multiple-reaction-mode (MRM).ResultsThe method established in this assay was successfully applied to the pharmacokinetic study of the selected coumarins in rat plasma after oral administration of the extract of ADR, and the pharmacokinetic characteristics of sixteen coumarins were clearly elucidated.ConclusionThis pharmacokinetic identification of multiple coumarins of ADR in rats provides a significant basis for better understanding the metabolic mechanism of the herb medicine.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 23, Issue 10, 15 September 2016, Pages 1029–1036
نویسندگان
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