کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496369 1116130 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of pulmonary metastasis by Emilia sonchifolia (L.) DC: An in vivo experimental study
ترجمه فارسی عنوان
مهار متاستاز ریوی توسط Emilia sonchifolia (L.) DC: یک مطالعه تجربی داخل بدن
کلمات کلیدی
Emilia sonchifolia؛ ملانوما B16F10؛ سیتوکین ها؛ فاکتور رشد اندوتلیال عروق؛ ماتریکس متالوپروتئیناز؛ مهار کننده بافت ماتریکس متالوپروتئیناز Erk، کیناز تنظیم شده با سیگنال خارج سلولی؛ GGT، گاما گلوتامیل ترانسپپتیداز؛ GM-CSF، granulocyte m
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی

BackgroundEmilia sonchifolia (L.) DC is a widely distributed medicinal herb used mainly in the indigenous Ayurvedic system of medicine in India. This plant is one among the ten sacred plants of Kerala state in India, collectively known as Dasapushpam.PurposeTo assess the therapeutic efficacy of this well-known medicinal plant in a catastrophic complication like metastatic cancer progression. This study further aimed to scientifically validate the traditional medicinal use of this sacred plant.Study designHighly metastatic B16F10 melanoma will spontaneously metastasize in C57BL/6 mice and is accepted as a useful murine model for the study on metastasis. Three different experimental modalities of prophylactic, simultaneous and after tumour development were used for data accumulation and analysis.MethodsWhole plant genuine extract of E. sonchifolia (25 mg/kg bodyweight) was administered intraperitoneally to C57BL/6 mice. Animals were sacrificed on 21st day after tumour induction and the lung tumour nodules were counted. Various lung and serum biochemical parameters along with major cytokine levels were recorded. Survival rate was monitored. Histopathology of the lung tissue and expression studies of the major genes involved in metastasis was also carried out.ResultsE. sonchifolia significantly inhibited pulmonary tumour formation and increased the life span of animals. Lung collagen hydroxyproline, uronic acid, hexosamine, serum sialic acid, γ-glutamyl transpeptidase, vascular endothelial growth factor (VEGF), granulocyte monocyte colony-stimulating factor and other cytokine levels were significantly lowered in the treated group of animals. Histopathological analysis was also correlated with these findings. E. sonchifolia down regulated the expression of matrix metalloproteinases; extracellular signal-regulated kinases and VEGF at the same time up regulated the expression of tissue inhibitor of matrix metalloproteinases.ConclusionPrevious studies on E. sonchifolia proved its significant biological properties including anti-tumour, anti-inflammatory and antioxidant activities. Present report is so far the first study to demonstrate the anti-metastatic potential of this medicinal herb justifying its conventional use in the traditional medicine.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 23, Issue 2, 15 February 2016, Pages 123–130
نویسندگان
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