کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496588 1116145 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Curcumin inhibits the SOS response induced by levofloxacin in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Curcumin inhibits the SOS response induced by levofloxacin in Escherichia coli
چکیده انگلیسی

The role of RecA protein in bacterial resistance to antibiotics makes this protein attractive from a pharmacological point of view. In this study we demonstrate that curcumin is able to inhibit the SOS response in Escherichia coli induced by levofloxacin. The blaTEM-1 gene has been placed under the control of the LexA-binding box and used as reporter gene. The expression of TEM-1 β-lactamase enzyme was increased in the presence of ssDNA induced by levofloxacin, while, the presence of curcumin at 8 μg/ml, reduced dramatically the expression of the reporter gene. Moreover a simple microplate assay suitable for high-throughput screening has been developed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 21, Issue 4, 15 March 2014, Pages 430–434
نویسندگان
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