کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496846 1116169 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro
چکیده انگلیسی

The antidiabetic activity of Momordica charantia (L.), Cucurbitaceae, a widely-used treatment for diabetes in a number of traditional medicine systems, was investigated in vitro. Antidiabetic activity has been reported for certain saponins isolated from M. charantia. In this study insulin secretion was measured in MIN6 β-cells incubated with an ethanol extract, saponin-rich fraction, and five purified saponins and cucurbitane triterpenoids from M. charantia, 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al (1), momordicine I (2), momordicine II (3), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-O-β-glucopyranoside (4), and kuguaglycoside G (5). Treatments were compared to incubation with high glucose (27 mM) and the insulin secretagogue, glipizide (50 μM). At 125 μg/ml, an LC–ToF-MS characterized saponin-rich fraction stimulated insulin secretion significantly more than the DMSO vehicle, p = 0.02. At concentrations 10 and 25 μg/ml, compounds 3 and 5 also significantly stimulated insulin secretion as compared to the vehicle, p ≤ 0.007, and p = 0.002, respectively. This is the first report of a saponin-rich fraction, and isolated compounds from M. charantia, stimulating insulin secretion in an in vitro, static incubation assay.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 19, Issue 1, 15 December 2011, Pages 32–37
نویسندگان
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