کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496863 1116170 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antidepressant-like effect of hyperoside isolated from Apocynum venetum leaves: Possible cellular mechanisms
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Antidepressant-like effect of hyperoside isolated from Apocynum venetum leaves: Possible cellular mechanisms
چکیده انگلیسی

In the present work, we studied the possible cellular mechanisms of hyperoside isolated from Apocynum venetum leaves in corticosterone-induced neurotoxicity, using PC12 cells as a suitable in vitro model of depression. Cell viability was quantitated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The release amount of lactic dehydrogenase (LDH) and intracellular Ca2+ concentration were measured using kit and transcript abundances of brain-derived neurotrophic factor (BDNF) and cAMP response element binding protein (CREB) were determined by real-time RT-PCR.The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and lactic dehydrogenase (LDH) assays showed that 2.5, 5 and 10 μg/ml hyperoside or 10 μM fluoxetine (FLU) protected PC12 cells from the lesion induced by a 48 h treatment with 10 μM corticosterone. Fura-2/AM (acetoxymethyl ester) assays showed that 2.5, 5 and 10 μg/ml hyperoside or 10 μM FLU attenuated the intracellular Ca2+ overloading in PC12 cells induced by corticosterone. The transcript abundance of BDNF and CREB in PC12 cells was elevated upon hyperoside treatment. These results suggest that the possible cellular mechanisms of hyperoside antidepressant-like effect is a cytoprotective action related to elevation the expression of BDNF and CREB through the signal pathway AC–cAMP–CREB.

We studied the possible cellular mechanisms of hyperoside isolated from Apocynum venetum leaves in corticosterone-induced neurotoxicity, using PC12 cells as a suitable in vitro model of depression. Cell viability was quantitated by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The release amount of lactic dehydrogenase (LDH) and intracellular Ca2+ concentration were measured using kit, and transcript abundances of brain-derived neurotrophic factor (BDNF) and cAMP response element binding protein (CREB) were determined by real-time RT-PCR.Figure optionsDownload high-quality image (93 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 19, Issue 2, 15 January 2012, Pages 145–149
نویسندگان
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