کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2497228 1556705 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of isoquinoline alkaloids from two Hippeastrum species on in vitro acetylcholinesterase activity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Effect of isoquinoline alkaloids from two Hippeastrum species on in vitro acetylcholinesterase activity
چکیده انگلیسی

The treatment of neurological disorders and neurodegenerative diseases is related to the levels of acetylcholine (ACh) through the inhibition of acetylcholinesterase (AChE). Galanthamine, an important alkaloid isolated from the Amaryllidaceae family, is approved for the pharmacological treatment of Alzheimer's disease (AD) and acts by inhibiting the acetylcholinesterase (AChE) activity. In the present study, Ellman's method was used to verify the inhibition of AChE activity of some isoquinolines alkaloids such as galanthamine, montanine, hippeastrine and pretazettine. At the concentrations 1 mM, 500 μm and 100 μm, galanthamine presented an AChE inhibition higher than 90%. Montanine inhibited, in a dose-dependent manner, more than 50% of the enzyme at 1 mM concentration. With the concentrations 500 μm and 100 μm, 30-45% of AChE activity inhibition was detected. The alkaloids hippeastrine and pretazettine presented no significant inhibition of the AChE activity. The results demonstrate that montanine significantly inhibits AChE activity at the tested concentrations, suggesting the necessity of further investigations on this alkaloid use in treating neurological disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 17, Issues 8–9, July 2010, Pages 698–701
نویسندگان
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