کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2497630 | 1116212 | 2010 | 6 صفحه PDF | دانلود رایگان |

A dimeric 64-kDa hemagglutinin was isolated with a high yield from dried Phaseolus vulgaris cultivar “French bean number 35” seeds using a chromatographic protocol that involved Blue-Sepharose, Q-Sepharose, and Superdex 75. The yield was exceptionally high (1.1 g hemagglutinin per 100 g seed), which is around 10–85 times higher than other Phaseolus cultivars. Its N-terminal sequence resembled those of other Phaseolus hemagglutinins. The hemagglutinating activity of the hemagglutinin was stable in the pH range 6–8, and in the temperature range 0 °C–50 °C. It inhibited HIV-1 reverse transcriptase with an IC50 of 2 μM. It suppressed mycelial growth in Valsa mali with an IC50 of 10 μM. It inhibited proliferation of hepatoma HepG2 cells and breast cancer MCF-7 cells with an IC50 of 100 and 2 μM, respectively. It had no antiproliferative effect on normal embryonic liver WRL68 cells.
Journal: Phytomedicine - Volume 17, Issue 6, May 2010, Pages 457–462