کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2497630 1116212 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of a French bean hemagglutinin with antitumor, antifungal, and anti-HIV-1 reverse transcriptase activities and an exceptionally high yield
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Isolation and characterization of a French bean hemagglutinin with antitumor, antifungal, and anti-HIV-1 reverse transcriptase activities and an exceptionally high yield
چکیده انگلیسی

A dimeric 64-kDa hemagglutinin was isolated with a high yield from dried Phaseolus vulgaris cultivar “French bean number 35” seeds using a chromatographic protocol that involved Blue-Sepharose, Q-Sepharose, and Superdex 75. The yield was exceptionally high (1.1 g hemagglutinin per 100 g seed), which is around 10–85 times higher than other Phaseolus cultivars. Its N-terminal sequence resembled those of other Phaseolus hemagglutinins. The hemagglutinating activity of the hemagglutinin was stable in the pH range 6–8, and in the temperature range 0 °C–50 °C. It inhibited HIV-1 reverse transcriptase with an IC50 of 2 μM. It suppressed mycelial growth in Valsa mali with an IC50 of 10 μM. It inhibited proliferation of hepatoma HepG2 cells and breast cancer MCF-7 cells with an IC50 of 100 and 2 μM, respectively. It had no antiproliferative effect on normal embryonic liver WRL68 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 17, Issue 6, May 2010, Pages 457–462
نویسندگان
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