کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2497776 | 1116223 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective](/preview/png/2497776.png)
The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS−, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS− at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction.
Journal: Phytomedicine - Volume 13, Issue 8, 11 September 2006, Pages 591–595