کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2497876 1116233 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China
چکیده انگلیسی

Radix Bupleuri (Chaihu), a famous Traditional Chinese Medicine, is derived from the dried roots of Bupleurum chinense DC. and B. scorzonerifolium Willd. But nowadays, other nine species and varieties from genus Bupleurum are also used as Radix Bupleuri in northwestern China. The authentic identification of dried roots of B. chinense and B. scorzonerifolium, however, is difficult to just base on the appearance and morphology. A molecular genetic method was developed to help discriminate the original species of Radix Bupleuri. The ITS sequences (∼600 bp) were amplified by the PCR technique from genomic DNA isolated from all the collected samples. According to analyzing the information given by ITS sequences, the conclusion can be made that ITS sequence could serve as markers for authentication of Radix Bupleuri.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 14, Issue 6, 27 June 2007, Pages 416–423
نویسندگان
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