کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2497951 1116245 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tissue distribution and excretion of resveratrol in rat after oral administration of Polygonum cuspidatum extract (PCE)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Tissue distribution and excretion of resveratrol in rat after oral administration of Polygonum cuspidatum extract (PCE)
چکیده انگلیسی

PurposePolygonum cuspidatum extract as a traditional Chinese medicine is extracted from the dried rhizome and root of Polygonum cuspidatum Sieb.et Zucc. Resveratrol is one of its active components. Studies were performed in rats to define the tissue distribution and excretion of resveratrol in urine and bile, and to characterize (if possible) any metabolites of resveratrol observed in tissues after ig 20 mg/kg Polygonum cuspidatum extract.MethodFor tissue distribution studies, tissues (300 mg) were homogenized and centrifuged with methanol, and metabolites found in selected tissue extract were identified by LC/MS/MS. For urinary and biliary excretion experiments, urine and bile samples were cleaned up by using solid-phase extraction (SPE) with polyamide cartridges. All the concentrations of resveratrol in these biological samples were determined by HPLC with UV detection.ResultAfter a single oral dose of 20 mg/kg PCE in rats, resveratrol was mainly distributed in stomach, duodenum, liver and kidney with detectable metabolites resveratrol monoglucuronide and resveratrol monosulfate. The majority of the resveratrol was excreted as metabolites, only 0.59% and 0.027% of the dosage were excreted in urine and bile respectively as unchanged drug within 24 h.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 15, Issue 10, 17 October 2008, Pages 859–866
نویسندگان
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