کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2526560 | 1558038 | 2013 | 8 صفحه PDF | دانلود رایگان |
AimTo investigate whether diosgenin could modulate tissue factor (TF) procoagulation activity, expression, and related signal transduction pathways.MethodsHuman THP-1 monocytic cells were exposed to tumor necrosis factor-α (TNF-α, 10 ng·mL−1) with or without diosgenin (0.01, 0.1, and 1 μmol·L−1) for 2 h or 5 h to induce TF procoagulant activity and expression, which were determined by the simplified chromogenic assay, reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative PCR, and Western blotting assays. In addition, the activation of the NF-κB, Akt, and MAPK signaling pathways were also measured by Western blotting.ResultsDiosgenin significantly inhibited TNF-α-induced TF procoagulant activity at concentrations of 0.01 to 1 μmol·L−1 with IC50 of 0.25 μmol·L−1. It also reduced protein expression and mRNA accumulation of TF dose-dependently in activated THP-1 cells. TNF-α stimulated significantly phosphorylation on Ser536 of NF-κB/p65, Ser473 of Akt at 5–15 min, and activations of IKK-β and ERK at 15–30 min. Diosgenin (1 μmol·L−1) could inhibit the phosphorylation of NF-κB/p65, IKK-β, Akt, ERK, and JNK, but had no remarkable effects on IκB and p38 phosphorylation in THP-1 cells.ConclusionDiosgenin inhibits TNF-α-induced TF activity and expression in monocytes, partly due to its down-regulation of the phosphorylation of NF-κB/p65, IKK-β, Akt, ERK, and JNK.
Journal: Chinese Journal of Natural Medicines - Volume 11, Issue 6, November 2013, Pages 608-615