کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2526609 1558051 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and Determination of Absorbed Components of Danggui-Shaoyao-San in Rat Plasma
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی طب مکمل و جایگزین
پیش نمایش صفحه اول مقاله
Identification and Determination of Absorbed Components of Danggui-Shaoyao-San in Rat Plasma
چکیده انگلیسی

AimTo investigate the absorbed components of Danggui-Shaoyao-San (DSS) in rat plasma and their pharmacokinetics.MethodsSerum samples from rats with DSS extract administrated orally were analyzed by HPLC-DAD-MSn and an HPLC-DAD method was established for the determination.ResultsSeven prototypes and one metabolite were identified in rat serum, and albiflorin, paeoniflorin, ferulic acid and ligustilide in rat plasma were determined for pharmacokinetic study. The proposed method was validated with a linear range of 0.025–5.60 μg·mL−1 and limit of quantitation (LOQ) at 0.022–0.180 μg·mL−1, respectively. The RSD of intra- and inter-day precision variations were less than 10.1% and the accuracies ranged from 92.5%–111.5%. The overall recovery was over 80%.ConclusionPaeoniflorin sulfonate, albiflorin, paeoniflorin, ferulic acid, senkyunolide I, ligustilide and butylidenephthalide were found to be the potentially effective constituents in DSS. The method for determination is simple and sensitive, which is proved to be suitable for pharmacokinetic study of albiflorin, paeoniflorin, ferulic acid and ligustilide in DSS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Natural Medicines - Volume 9, Issue 5, September 2011, Pages 363-368