Analysis of Secoiridoid Glucosides in Jasminum lanceolarium Roxb. by HPLC-MS

AimTo develop high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) for the analysis and identification of secoiridoid glucosides in Jasminum lanceolarium Roxb.MethodsThe ultraviolet (UV) chromatogram, total ion current chromatogram (TIC) and extracted ion chromatogram (EIC) of the sample and ESI/MS/MS spectra of the peaks in the chromatograms were obtained in order to identify the structure of secoiridoid glucosides.ResultsThe six identified secoiridoid glucosides were Jasminoside, Jaslanceoside A, Jaslanceoside B, Jaslanceoside C, Jaslanceoside D and Jaslanceoside E. The structures of another four unknown secoiridoid glucosides were speculated.ConclusionThe developed simple and rapid method was useful to isolate the new secoiridoid glycoside from Jasminum lanceolarium quickly and to authenticate the species of the herb.