Flavonoids from the bran of Avena sativa

AimTo investigate the chemical constituents from the bran of Avena sativa.MethodsCompounds were isolated and purified by various column chromatographic techniques using macroporous resin, silica gel, Sephadex LH-20, ODS, and preparative reversed-phase HPLC. Their structures were elucidated by physicochemical properties and spectroscopic data.ResultsFifteen chemical constituents were isolated and identified as kaempferol 3-O-(2″, 3″-di-E-p-coumaroyl)-a-L-rhamnopyranoside (1), kaempferol 3-O-(3″-E-p-coumaroyl)-a-L-rhamnopyranoside (2), kaempferol 3-O-(2″-O-E-p-coumaroyl)-β-D-glucopyranoside (3), kaempferol 3-O-β-D-glucopyranoside (4), kaempferol 7-O-a-L-rhamnopyranoside (5), linarin (6), tilianin (7), myricitrin (8), quercitrin (9), kaempferol 3-O-rutinoside (10), rutin (11), tricin 7-O-β-D-glucopyranoside (12), tricin (13), kaempferol (14), and luteolin (15).ConclusionCompounds 1-9 were isolated from Avena sativa Linn. for the first time.