DPPH Radical Scavenging Activity and Total Phenolics of Phellinus Mushroom Extracts Collected from Northeast of Thailand

AimTo investigate the antioxidant activity and total phenolic content from the crude extracts and crude fractions of ten species of Phellinus mushrooms collected from northeast Thailand.MethodsThe samples were tested for their radical scavenging activity toward 2, 2-diphenyl-1-pricylhydrazyl (DPPH) radical (DPPH method) and total phenolic content (Folin-Ciocalteu method).ResultsSome of the investigated extracts exhibited potent radical scavenging activity with the IC50 ranging from (7.30 ± 0.34) to (19.80 ± 0.13) μg·mL−1. IC50 were in the range of the standard antioxidant used; quercetin, ascorbic acid and butylated hydroxytoluene (BHT). The strongest scavenging activity as comparable to quercetin was found in the crude 80% EtOH extract of P. torulosus. The crude EtOAc fraction of P. pini showed the highest total phenolic content with a value of 87.76 ± 1.00 equivalent gallic acid (EGA) while the samples with potent antioxidant activity were also determined to have high amount of total phenolics (78.34 ± 0.27 to 51.01 ± 0.38) EGA.ConclusionThese results indicated that crude extracts from Phellinus mushrooms have a potential to be natural antioxidant source.