کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2531629 1558941 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Neuropeptide FF receptors exhibit direct and anti-opioid effects on mice dorsal raphe nucleus neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Neuropeptide FF receptors exhibit direct and anti-opioid effects on mice dorsal raphe nucleus neurons
چکیده انگلیسی

By using acutely dissociated dorsal raphe nucleus neurons (DRN) from young mice, direct and anti-opioid effects of Neuropeptide FF (NPFF) receptors were measured. The NPFF analog 1DMe (10 µM) had no effect on resting Ca2+ channels but reduced the magnitude of Ca2+ transients induced by depolarization in 83.3% neurons tested, of which the inhibition rate is 45.4±2.9%. Pertussis toxin treatment reduced to 18.9% the number of responding neurons and attenuated by 47% the response of 1DMe. In contrast, cholera toxin treatment had no significant effect. Eighteen minute perfusion with 1DMe at a very low 10 nM concentration, that did not directly inhibit Ca2+ transients triggered by depolarization in every neuron, attenuated by 78% the inhibitory effect of Nociceptin/orphanin FQ (N/OFQ) on Ca2+ transients, but not that of by serotonin. These results demonstrated for the first time that NPFF receptors on mice DRN inhibit Ca2+ transients induced by depolarization via Gi/o protein and also exhibit a specific anti-opioid activity on nociceptin receptors, and that their specific anti-opioid activity is not a direct consequence of their activity on Ca2+ transients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 740, 5 October 2014, Pages 271–276
نویسندگان
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