Activity of antibacterial, antiviral, anti-inflammatory in compounds andrographolide salt

Andrographolide sulfonic acid sodium salt (ASS) was synthesized to increase the the solubility of Andrographolide in aqueous solution. We have studied its pharmacological effect of antibiosis, anti-inflammatory and immunoregulation. Cylinder-plate method was used to study ASS׳s in vitro antibacterial activity, and its protection for mice infected by Staphylococcus aureus and Shigella dysenteriae. Various inflammation models, including the auricular edema induced by xylene in mice, CMC-Na induced air pounch model and the paw edema induced by albumen in rats were used to explore the characteristic of ASS׳s anti-inflammation effect. We built up the immune model by injecting chicken red cells in enter celiac of mice and study the effect of ASS on immunoregulation, taking andrographolide as the positive control. bacteriostasis in vivo and in vitro experiments show that ASS has a weak antibacterial effect and no bactericidal effect, but can reduce the mice mortality of Staphylococcus aureus infected. Anti-inflammatory experiments show that ASS can reduce the mouse ear swelling induced by xylene and rat paw swelling induced by egg albumin, and lessen leukocytes in air bag caused by CMCNa, and lower IL1 not ably in rat serum. Immune tests indicate that ASS can get spleen and thymus gain weight and increase rate of abdominal macrophage phagocytosis of mice. The result of bacteriostasis shows that ASS has weak in vitro antibacterial effect. ASS shows significant effects of anti-inflammation and improving immunity, thus enables the mice against bacteria better.

Andrographolide is the main active ingredient in the common Andrographis paniculata (Burm. f.) Nees which is commonly used in chinese patent medicine against inflammatory. It can relieve internal fever, detoxicate, diminish inflammation, stop pain and has good curative effect to the upper respiratory tract infection and diarrhea by bacterial and virus. Andrograph- olide is a kind of diterpenes lactone and indissolvable in water, so only oral administration can be taken. Anti-inflammatory activities of Andrographolide sulfonic acid sodium salt (ASS) which was developed from andrographolide was evaluated , with andrographolide as the positive control.Figure optionsDownload high-quality image (196 K)Download as PowerPoint slide