کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2533025 | 1559040 | 2011 | 10 صفحه PDF | دانلود رایگان |
We characterized agonist-induced internalization, recycling and downregulation of each muscarinic receptor subtype (M1–M5) stably expressed in Chinese hamster ovary (CHO) cells. The radioligands [3H]QNB and [3H]NMS were used to measure the total and plasma membrane populations of muscarinic receptors, respectively. Following carbachol treatment (1 mM), the rank orders for the rate of carbachol-induced internalization of the muscarinic subtypes were M2 > M4 = M5 > M3 = M1, respectively. Unlike the M2 receptor, M1, M3, M4 and M5 receptors recycled back to the plasma membrane after 1 h carbachol treatment. The receptor downregulation elicited to 24 h carbachol treatment was similar for M2, M3, M4 and M5 receptors, whereas that for the M1 receptor was greater. Our results indicate that there are subtype-specific differences in the rate and extent of agonist-induced muscarinic receptor internalization, recycling and downregulation in CHO cells.
Journal: European Journal of Pharmacology - Volume 650, Issues 2–3, 15 January 2011, Pages 534–543