کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2534120 | 1559077 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thrombin induces cyclooxygenase-2 expression via the ERK and NF-κB pathways in human lung fibroblasts
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Thrombin induces cyclooxygenase-2 expression via the ERK and NF-κB pathways in human lung fibroblasts Thrombin induces cyclooxygenase-2 expression via the ERK and NF-κB pathways in human lung fibroblasts](/preview/png/2534120.png)
چکیده انگلیسی
There is growing evidence that increased expression of cyclooxygenase-2 (COX-2) in the lungs of patients is a key event in the pathogenesis of lung diseases. In this study, we investigated the involvement of the extracellular signal-regulated kinase (ERK), IκB kinase α/β (IKKα/β), and nuclear factor-κB (NF-κB) signaling pathways in thrombin-induced COX-2 expression in human lung fibroblasts (WI-38). Treatment of WI-38 cells with thrombin caused increased COX-2 expression in a concentration- and time-dependent manner. Treatment of WI-38 cells with PD 98059 (2-[2-amino-3-methoxyphenyl]-4H-1-benzopyran-4-one, a MEK inhibitor) inhibited thrombin-induced COX-2 expression and COX-2-luciferase activity. Stimulation of cells with thrombin caused an increase in ERK phosphorylation in a time-dependent manner. In addition, treatment of WI-38 cells with Bay 117082, an IκB phosphorylation inhibitor, and pyrrolidine dithiocarbamate (PDTC), an NF-κB inhibitor, inhibited thrombin-induced COX-2 expression. The thrombin-induced increase in COX-2-luciferase activity was also blocked by the dominant negative IκBα mutant (IκBαM). Treatment of WI-38 cells with thrombin induced IKKα/β and IκBα phosphorylation, IκBα degradation, and κB-luciferase activity. The thrombin-mediated increases in IKKα/β phosphorylation and κB-luciferase activity were inhibited by PD 98059. Taken together, these results suggest that the ERK-dependent IKKα/β/NF-κB signaling pathway plays an important role in thrombin-induced COX-2 expression in human lung fibroblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 618, Issues 1â3, 15 September 2009, Pages 70-75
Journal: European Journal of Pharmacology - Volume 618, Issues 1â3, 15 September 2009, Pages 70-75
نویسندگان
Chung-Huang Shih, Mauo-Ying Bien, Ling-Ling Chiang, Chien-Ling Su, Chien-Huang Lin, Bing-Chang Chen,