کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2534213 | 1559080 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A cembranoid from tobacco prevents the expression of nicotine-induced withdrawal behavior in planarian worms A cembranoid from tobacco prevents the expression of nicotine-induced withdrawal behavior in planarian worms](/preview/png/2534213.png)
Using an adaptation of published behavioral protocols, we determined that acute exposure to the cholinergic compounds nicotine and carbamylcholine decreased planarian motility in a concentration-dependent manner. A tobacco cembranoid (1S,2E,4R,6R,7E,11E)-cembra-2,7,11-triene-4,6-diol (4R-cembranoid), also decreased planarian motility. Experiments in the presence of 1 µM 4R-cembranoid did increase the IC50 for nicotine- but not carbamylcholine-induced decrease in planarian motility. When planarians were exposed for 24 h to either nicotine or carbamylcholine at concentrations near their respective IC50 values and then transferred to plain media, nicotine-exposed, but not carbamylcholine- or cembranoid-exposed worms displayed withdrawal-like distress behaviors. In experiments where planarians were pre-exposed to 100 µM nicotine for 24 h in the presence of 1 µM 4R-cembranoid, the withdrawal-like effects were significantly reduced. These results indicate that the 4R-cembranoid might have valuable applications for tobacco abuse research. This experimental approach using planarians is useful for the initial screening of compounds relevant to drug abuse and dependence.
Journal: European Journal of Pharmacology - Volume 615, Issues 1–3, 1 August 2009, Pages 118–124