کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2534335 | 1559085 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and functional evidence of the prostaglandin F2α receptor mediating contraction in human umbilical vein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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![عکس صفحه اول مقاله: Expression and functional evidence of the prostaglandin F2α receptor mediating contraction in human umbilical vein Expression and functional evidence of the prostaglandin F2α receptor mediating contraction in human umbilical vein](/preview/png/2534335.png)
چکیده انگلیسی
Our purposes were to perform the pharmacological characterization of PGF2α receptor (prostanoid FP-receptor) involved in human umbilical vein contraction and confirm its expression in this tissue. Umbilical cords from healthy patients after full-term deliveries were employed. The vein was dissected out of cords and used for either isolated organ bath or reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assays. The natural prostanoid FP-receptor agonist, PGF2α, and its selective analogues, latanoprost and bimatoprost free acids are full agonists (produce more than 80% of the maximal contractile response to 5-HT) in human umbilical vein. The agonist potency (pEC50) order was PGF2α (6.01 ± 0.05) > latanoprost free acid (5.65 ± 0.07) = bimatoprost free acid (5.59 ± 0.08). The contractile effects of PGF2α and latanoprost free acid were blocked competitively by the prostanoid FP-receptor antagonist, AL-8810. The antagonist potencies (pKB) of AL-8810 vs. PGF2α (5.93 ± 0.05) and vs. latanoprost free acid (6.40 ± 0.08) in human umbilical vein are in good agreement with its ability to antagonize prostanoid FP receptors of rat, mouse and human cells. In all samples, clear signal was detected for cDNA amplification of prostanoid FP receptor and the specific prostanoid FP-receptor antibody recognized a protein of approximately 64 kDa. In conclusion, taking into account the obtained functional and biochemical data, we propose for the first time that human umbilical vein express prostanoid FP-receptors and these receptors could be involved in the vasoconstriction action of PGF2α in this tissue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 610, Issues 1â3, 21 May 2009, Pages 68-74
Journal: European Journal of Pharmacology - Volume 610, Issues 1â3, 21 May 2009, Pages 68-74
نویسندگان
Andrea Emilse Errasti, Gianina del-Rey, Cristián Emanuel Cesio, Guadalupe Souza, Wanda Nowak, Facundo Germán Pelorosso, Federico Manuel Daray, Rodolfo Pedro Rothlin,