کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2535306 | 1559117 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anion secretion evoked by Pasteurella multocida toxin across rat colon
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Anion secretion evoked by Pasteurella multocida toxin across rat colon Anion secretion evoked by Pasteurella multocida toxin across rat colon](/preview/png/2535306.png)
چکیده انگلیسی
Stimulation of muscarinic receptors is known to have a biphasic effect on colonic Clâ secretion: a short-lasting activation, which is followed by a long-lasting inhibition. In order to find out, which role Gq proteins play in both processes, Pasteurella multocida toxin was used, a known activator of Gαq. This toxin (1.5 µg/ml) had a dual action on short-circuit current (Isc) across rat distal colon: it stimulated transiently Isc and subsequently down-regulated the Isc evoked by Ca2+-dependent secretagogues such as acetylcholine or ATP. The inactive mutant (P. multocida toxin C1165S), which does not stimulate Gαq, was ineffective. Clâ dependence and sensitivity against bumetanide, a blocker of the Na+-K+-2Clâ cotransporter, confirmed that the increase in Isc evoked by the toxin represented Clâ secretion. The effect of P. multocida toxin was suppressed by YM-254890 (10â 7 M), a blocker of Gαq. Experiments with apically permeabilized tissues revealed that the secretory response to P. multocida toxin was concomitant with an increase in basolateral K+ conductance as it is observed for other agonists inducing Ca2+-dependent anion secretion. Consequently, these results suggest that Gq proteins are not only involved in the activation of secretion, e.g. after stimulation of muscarinic or purinergic receptors, but also play a central role in the long-term down-regulation of intestinal secretion after activation of these types of receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 583, Issue 1, 31 March 2008, Pages 156-163
Journal: European Journal of Pharmacology - Volume 583, Issue 1, 31 March 2008, Pages 156-163
نویسندگان
Britta Hennig, Joachim Orth, Klaus Aktories, Martin Diener,