کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2537137 | 1559180 | 2006 | 8 صفحه PDF | دانلود رایگان |
In cardiovascular diseases, endothelin type B (ETB) receptors in arterial smooth muscle cells are upregulated. The present study revealed that organ culture of rat mesenteric artery segments enhanced endothelin ETB receptor-mediated contraction paralleled with increase in the receptor mRNA and protein expressions. The endothelin ETB receptor-mediated contraction was associated with increase in phosphorylation of extracellular regulation kinase 1 and 2 (ERK1/2) proteins and elevated levels of intracellular calcium. The elevation curve of intracellular calcium consisted of two phases: one rapid and one sustained. Inhibition of ERK1/2 phosphorylation by SB386023 or blockage of calcium channels by nifedipine significantly reduced the endothelin ETB receptor-mediated contraction (P < 0.05) and decreased the sustained phase of intracellular calcium level, but not the rapid phase. Thus, phosphorylation of ERK1/2 proteins and elevation of intracellular calcium level are required for endothelin ETB receptor-mediated contraction in rat mesenteric artery.
Journal: European Journal of Pharmacology - Volume 538, Issues 1–3, 24 May 2006, Pages 124–131