کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2537162 | 1559183 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of a novel regulatory mechanism for norepinephrine transporter activity by the IP3 receptor Identification of a novel regulatory mechanism for norepinephrine transporter activity by the IP3 receptor](/preview/png/2537162.png)
The norepinephrine transporter (NET) plays a crucial role in noradrenergic neurotransmission and is a target of many antidepressants and psychostimulants. Intracellular Ca2+ is reportedly involved in regulating NET activity, but the detailed mechanism is not clear. We employed a norepinephrine uptake assay using SH-SY5Y cells and found that the IP3 receptor inhibitors, 2-aminoethoxydiphenyl borate and xestospongin C, reduced the NET Vmax. These reductions were accompanied by the decreased cell surface expression of NET. Our findings suggest that intracellular Ca2+ mobilized by IP3 receptor is required for the maintenance of NET activity. This adds another pathway involving Ca2+ for the regulation of NET to other known mechanisms providing intracellular Ca2+.
Journal: European Journal of Pharmacology - Volume 536, Issues 1–2, 24 April 2006, Pages 62–68