19(4 → 3)-abeo-abietane diterpenoids from Scrophularia dentata Royle ex Benth

Five 19(4 → 3)-abeo-abietane diterpenoids, scrodentoids A–E (1–5), were isolated from the whole plant of Scrophularia dentata. Planar structures of scrodentoids A–E were elucidated mainly by using 1D, 2D NMR and MS data. The absolute configurations of compounds 1 and 2 were established using X-ray crystallographic analysis. The absolute configurations of other compounds were confirmed using HPLC-UV/CD detection. The immunosuppressive effects of compounds 1–5 were studied using a ConA-induced splenocyte proliferation model. These compounds significantly inhibited ConA-induced splenocyte proliferation, with IC50 values in the range of 3.49–133.86 μM. Compounds 1–5 (IC50 > 10 μM) showed no discernible cytotoxic activity against B16 or MCF-7 cells.

Five 19(4 → 3)-abeo-abietane diterpenoids were isolated from the whole plant of Scrophularia dentata. Among them, 2 showed the potential immunosuppressive effect with IC50 3.49 μM.Figure optionsDownload high-quality image (124 K)Download as PowerPoint slide