Anti-HBV activities of Streblus asper and constituents of its roots

The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper. The structures were elucidated by spectroscopic methods, including 1D NMR (1H NMR, 13C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1–3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC50 values of 3.14 μM and 4.74 μM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC50 values of 11.81 μM and 25.80 μM for HBsAg and HBeAg respectively.

The extracts of S. asper and some isolates were investigated for anti-HBV activities. A novel compound, (7R,8S,7'R,8'S)-erythro-strebluslignanol H, was isolated from of the roots of S. asper.Figure optionsDownload high-quality image (33 K)Download as PowerPoint slide