کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2538845 | 1122157 | 2011 | 5 صفحه PDF | دانلود رایگان |
Rheum undulatum root has been used traditionally in Korea for the treatment of dental diseases. The purpose of this study was to separate a fraction from R. undulatum showing anti-acid production activity against Streptococcus mutans biofilms and identify the main components in that fraction. Methanol extract of R. undulatum root and its fractions were prepared. To select a fraction exhibiting anti-acid production activity, suspension glycolytic pH-drop assay was performed. Among the fractions tested, dichloromethane fraction exhibited the strongest activity in a dose-dependent manner. To examine the effect of the selected fraction on the anti-acid production of S. mutans biofilms, 74 h old S. mutans biofilms were used. The selected fraction reduced the initial rate of acid production of S. mutans biofilms at sub-minimum inhibitory concentration (MIC) levels. HPLC qualitative analysis of the selected fraction indicated that the presence of anthraquinone derivatives, such as aloe-emodin, emodin, chrysophanol and physcion, as main components.
Graphical AbstractDichloromethane fraction from Rheum undulatum root, mainly composed of aloe-emodin, emodin, chrysphanol and physcion, showed inhibitory effect on the acid production of Streptococcus mutans biofilms at sub-minimum inhibitory concentration (MIC) levels.Figure optionsDownload as PowerPoint slide
Journal: Fitoterapia - Volume 82, Issue 3, April 2011, Pages 352–356