Combinatorial search for the ligands that specifically recognize the streptococcal collagen-like proteins Scl1 and Scl2

Phage-encoded peptide libraries are often used to study biomolecular interactions. We employed this combinatorial approach to identify ligands specific for the streptococcal collagen-like proteins, Scl1 and Scl2, which are expressed on the cell surface by group A Streptococcus. Several sequence motifs displayed by phages were selected for their binding ability to different Scl variants.