کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2584827 1561747 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro investigations of gold nanocages: Toxicological profile in human keratinocyte cell line
ترجمه فارسی عنوان
تحقیقات در شرایط آزمایشگاهی از نانوقفسه طلا: مشخصات سم شناسی در سلول کراتینوسیت انسانی
کلمات کلیدی
نانوقفسه طلا؛ چرخه سلولی؛ سلول کراتینوسیت انسانی؛ جذب سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


• Gold nanocages cause a cell cycle arrest at G2/M phase in Human keratinocytes.
• Concentration, incubation time and surface modification of gold nanocages are used as variables.
• G2/M phase arrest leads towards apoptosis in Human keratinocytes.

Gold nanocages (AuNCs) are comparatively novel nanostructures, as many of their characteristics are still to be exploited. The purpose of present study was to systematically investigate the toxicological effects of AuNCs on human keratinocyte cell line (HaCaT) utilizing Dark Field (DF)/Bright Field (BF) imaging and flow cytometry cell cycle techniques. We have applied surface modification, concentration, and incubation time of AuNCs as variables to investigate their effect on the cellular imaging and cell cycle response of HaCaT cells. The results indicate that the AuNCs interact with HaCaT cells in accordance to their surface charge and concentration. Cellular uptake is evident from DF images which lead to the cell cycle perturbations and apoptosis in HaCaT cells. AuNCs cause a prominent G2/M phase arrest after 24 h of incubation. To the best of our knowledge toxicological effects of AuNCs on cell cycle of HaCaT cell line in vitro are not reported previously.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 97, November 2016, Pages 89–95
نویسندگان
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