کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2585185 1561783 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation of in vivo toxicity of hydroxylamine sulfate and the efficiency of intoxication treatment by α-tocopherol acetate and methylene blue
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Investigation of in vivo toxicity of hydroxylamine sulfate and the efficiency of intoxication treatment by α-tocopherol acetate and methylene blue
چکیده انگلیسی


• Hydroxylamine sulfate cause oxidative stress and MetHb, Hb–NO formation.
• Stable complex of reduced iron (Fe2+) with hydroxylamine sulfate was identified.
• Methylene blue does not reduce the levels of MetHb in case of HAS intoxication.
• Methylene blue does not reduce the levels of Hb–NO in case of HAS intoxication.
• Methylene blue is not suitable antidote in case of hydroxylamine intoxication.

ObjectivesInvestigation of hydroxylamine sulfate toxicity mechanism in vivo and estimation of α-tocopherol acetate and methylene blue efficiency in poisoning treatments.MethodsIn vivo experiments were conducted on 102 Wistar Han rats. The experiments investigated the hematotoxic and oxidative stress effects of hydroxylamine sulfate in acute and subacute toxicity treatment of animals. Electron Spin Resonance was used for quantitative determination of blood and liver tissue parameters alterations after intoxication. The osmotic fragility of erythrocytes, lipid peroxidation intensity and level of SH-groups in liver of rats were determined by established biochemical assays.ResultsHydroxylamine sulfate cause an acute hematotoxicity and oxidative stress in vivo as demonstrated by the appearance of free oxidized iron in blood, reduced glutathione content and increased lipid peroxidation in liver. The experimental studies showed the formation of Hb–NO, MetHb in erythrocytes and as well of stable complex of reduced iron (Fe2+) with hydroxylamine sulfate. Methylene blue treatment does not reduce the Hb–NO or MetHb levels in intoxicated animals while administration of α-tocopherol acetate reduces substantially lipid peroxidation.ConclusionsOxidative stress is a key mechanism of acute hematotoxicity caused by hydroxylamine sulfate. Methylene blue is not suitable antidote in case of hydroxylamine intoxication.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 61, November 2013, Pages 227–232
نویسندگان
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