کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2587664 1130935 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of cell death in RAW 264.7 cells by alpha-lactalbumin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Induction of cell death in RAW 264.7 cells by alpha-lactalbumin
چکیده انگلیسی

Alpha-lactalbumin (α-LA), a major human milk whey protein, has been reported to exhibit bactericidal properties, immune suppressive effects, anti-proliferation and apoptosis in transformed cells; however, little is known about its anti-inflammation and related molecular mechanism. In this study we investigated the effects of α-LA on macrophages. We found that treatment with high concentration α-LA (≥100 μg/ml) could result in a time- and dose-dependent decrease in growth activity, morphological changes, increase in hypodiploid DNA population, and DNA fragmentation in RAW 264.7 cells. We also found that high dose α-LA could induce cellular apoptosis and necrosis, as determined by Annexin V binding assay. The α-LA could enhance the expression levels of cytochrome c, active caspase 3, active caspase 8, extracellular signal-regulated kinase (ERK1/2) and c-Jun N-terminal kinase (JNK) activation without changing the protein levels, but suppress the protein level of Bcl-2. The broad-spectrum caspase inhibitor, Boc-D-fmk, failed to block cell death, indicating that α-LA-induced cell death was modulated in a caspase-independent manner. In addition, the ERK1/2 inhibitor, PD98059, could partially rescue α-LA-induced cell death, while the JNK inhibitor, SP600125, could weakly protect cells from death. Our results suggested that activation of ERK1/2 might mediate α-LA-induced cell death in RAW 264.7 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 46, Issue 3, March 2008, Pages 842–853
نویسندگان
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