کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2588169 | 1130955 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evaluation of estrogenic activity of organic biocides using ER-binding and YES assay Evaluation of estrogenic activity of organic biocides using ER-binding and YES assay](/preview/png/2588169.png)
Biocides have been used not only in everyday items such as clothes, kitchenware, daily necessities, and infant utensils, but also in cosmetics and wrapping papers for foodstuffs. Since there is a high possibility of exposure to biocides from such materials, their safety must be assessed adequately using a range of methods. We investigated the estrogenic activity of 20 organic biocides using two in vitro screen assays: estrogen receptor (ER) binding assay and yeast estrogen screen (YES) assay. Twelve of the biocides were positive in the ER-binding assay. Regardless of the presence or absence of rat S9Mix for metabolic activation, 4-chloro-3-methylphenol was positive in both ER-binding and YES assay. 4-Chloro-3,5-dimethylphenol was positive in the ER-binding assay and showed a pseudopositive manner in the YES assay that was observed the dose-independent estrogenic activity at only one dose point. Hiba oil was positive in the ER-binding assay but was positive in the YES assay only in the presence of rat S9Mix. These results suggest that ER-binding and YES assay could be adapted for evaluation of the endocrine-disrupting activity of biocides. The biocides found to be positive in vitro now require assessment by in vivo screening methods.
Journal: Food and Chemical Toxicology - Volume 45, Issue 9, September 2007, Pages 1558–1564