کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2705991 1144790 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficient Transduction of 11 Poly-arginine Peptide in an Ischemic Lesion of Mouse Brain
ترجمه فارسی عنوان
ترانسفوزیون کارآمد 11 پپتید پلی-آرژینین در یک بیماری اسکیزوفرنی مغز موش
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی مغز و اعصاب بالینی
چکیده انگلیسی

Direct intracellular delivery of intact proteins has been successfully achieved by tagging cell-penetrating peptide (CPP), which consists of short positively charged amino acids, such as 11 poly-arginine (11R); however, in vivo delivery of the proteins to the brain has remained challenging because it is unclear whether CPP would enable proteins to cross the blood–brain barrier (BBB). In this study, we conducted an in vivo kinetic study to investigate the efficiency of 11R-mediated peptide delivery in the normal and ischemic brain. The 11R was observed in the microvessels and neurons surrounding the microvessels throughout the brain 1 hour after systemic administration, but the signal of the peptide was faint after 2 hours. In a transient middle cerebral artery occlusion mouse model, 11R was markedly enhanced and remained detectable in the cells on the ipsilateral side for as long as 8 hours after administration compared with the contralateral side. These results suggest that 11R is capable of in vivo delivery to the brain by passing through the BBB. Furthermore, 11R-mediated protein transduction could be used for the delivery of therapeutic molecules in cerebral ischemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Stroke and Cerebrovascular Diseases - Volume 23, Issue 8, September 2014, Pages 2023–2030
نویسندگان
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