کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2778068 | 1568045 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of protein kinase C in 15-HETE-induced hypoxic pulmonary vasoconstriction
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of the present study was to investigate the roles of protein kinase C (PKC) signal transduction pathway in the 15-hydroxyeicosatetraenoic acid (15-HETE)-induced down-regulation expression of KV 1.5, KV 2.1 and KV 3.4, and pulmonary vasoconstriction under hypoxia. Tension measurements on rat pulmonary artery (PA) rings, Western blots, semi-quantitative PCR and whole-cell patch-clamp technique were employed to investigate the effects of 15-HETE on PKC and KV channels. Hypericin (6.8 μmol/L), a PKC inhibitor, significantly attenuated the constriction of PA rings to 15-HETE under hypoxia. The down-regulation of KV 1.5, KV 2.1 and KV 3.4 channel expression and inhibition of whole-cell K currents (IKV) induced by 15-HETE were rescued and restored, respectively, by hypericin. These studies indicate that the PKC signal transduction pathway is involved in 15-HETE-induced rat pulmonary vasoconstriction under hypoxia. 15-HETE suppresses the expression of KV 1.5, KV 2.1 and KV 3.4 channels and inhibits IKV through the PKC signaling pathway in pulmonary arterial smooth muscle cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids - Volume 80, Issues 2â3, FebruaryâMarch 2009, Pages 115-123
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids - Volume 80, Issues 2â3, FebruaryâMarch 2009, Pages 115-123
نویسندگان
Lei Guo, Xiaobo Tang, Xiaojie Chu, Lihua Sun, Lei Zhang, Zhaoping Qiu, Shuo Chen, Yumei Li, Xiaodong Zheng, Daling Zhu,