کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2783512 1153751 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and functional characterization of DNA damage repair protein (DRT) from Lepidium latifolium L.
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Isolation and functional characterization of DNA damage repair protein (DRT) from Lepidium latifolium L.
چکیده انگلیسی

We have isolated and in silico characterized a cold regulated plastocyanin encoding gene from Lepidium latifolium L designated as LlaDRT. Its cDNA sequence (JN214346) consists of a 504 bp ORF, 48 and 205 bp of 5′ and 3′ UTR regions, respectively encoding a protein of 17.07 KDa and pI 4.95. In silico and phylogenetic analysis of LlaDRT suggested that the protein has features of a typical plastocyanin family member and of a nearest relative of the predominant isoform of Arabidopsis (PETE2) plastocyanin. Validation of stress response of LlaDRT by qPCR under different abiotic stress regulators viz salicylic acid, jasmonic acid, calcium chloride, ethylene and abscisic acid revealed its possible regulation and crosstalk amongst different pathways.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comptes Rendus Biologies - Volume 337, Issue 5, May 2014, Pages 302–310
نویسندگان
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