کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2791461 1154884 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alpha- and beta-keratins of the snake epidermis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Alpha- and beta-keratins of the snake epidermis
چکیده انگلیسی

Snake scales contain specialized hard keratins (beta-keratins) and alpha- or cyto-keratins in their epidermis. The number, isoelectric point, and the evolution of these proteins in snakes and their similarity with those of other vertebrates are not known. In the present study, alpha- and beta-keratins of snake molts and of the whole epidermis have been studied by using two-dimensional electrophoresis and immunocytochemistry. Specific keratins in snake epidermis have been identified by using antibodies that recognize acidic and basic cytokeratins and avian or lizard scale beta-keratin. Alpha keratins of 40–70 kDa and isoelectric point (pI) at 4.5–7.0 are present in molts. The study suggests that cytokeratins in snakes are acidic or neutral, in contrast to mammals and birds where basic keratins are also present. Beta keratins of 10–15 kDa and a pI of 6.5–8.5 are found in molts. Some beta-keratins appear as basic proteins (pI 8.2) comparable to those present in the epidermis of other reptiles. Some basic “beta-keratins” associate with cytokeratins as matrix proteins and replace cytokeratins forming the corneous material of the mature beta-layer of snake scales, as in other reptiles. The study also suggests that more forms of beta-keratins (more than three different types) are present in the epidermis of snakes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Zoology - Volume 110, Issue 1, 1 February 2007, Pages 41–47
نویسندگان
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