کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2794274 1155259 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential regulation of B-cell proliferation by IL21 in different subsets of chronic lymphocytic leukemia
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Differential regulation of B-cell proliferation by IL21 in different subsets of chronic lymphocytic leukemia
چکیده انگلیسی


• IL21 enhanced the proliferative effects of CpG in both normal and leukemic B cells.
• The combination of IL21 + CpG inhibited the proliferation of B-CLL cells from progressive patients.
• IL21 + CpG enhanced proliferation of B-CLL cells from IGHV mutated compared to unmutated patients.

Interleukin-21 (IL21) plays an important role in B-cell proliferation, survival and differentiation. Contrary to its stimulatory effect in normal B cells, it has been shown that it induces pro-apoptotic effect in leukemic B cells from CLL patients. Little is known regarding the biological function of IL21 in leukemic B cells from progressive and non-progressive CLL patients. In the present study, the proliferative effect of IL21 in combination with TLR9 agonist (CpG) was investigated in B cells isolated from 24 CLL patients and eight normal subjects by radioactive thymidine incorporation assay. B cells were enriched from peripheral blood mononuclear cells by negative selection using magnetic beads (MACS) and immunophenotyped by flow cytometry. Our results showed that IL21 enhanced the proliferative effects of CpG in both normal and leukemic B cells, though no significant differences were observed between CLL patients and healthy controls. Comparison between different subsets of patients revealed that while the combination of IL21 and CpG significantly inhibited the proliferation of B cells from progressive compared to non-progressive patients (p = 0.001), it enhanced proliferation of leukemic B cells from IGHV mutated compared to unmutated patients (p = 0.001). The inhibitory effect of IL21 on proliferation of normal and leukemic cells was found to be apoptosis-independent. Our findings suggest differential effects of IL21 in different subsets of CLL patients and suggest its potential therapeutic implication in patients with a more progressive disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 62, Issue 3, June 2013, Pages 439–445
نویسندگان
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