کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2794669 | 1155294 | 2011 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cloning, expression analysis and bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-22 Cloning, expression analysis and bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-22](/preview/png/2794669.png)
This report describes the cloning and characterisation of rainbow trout (Oncorhynchus mykiss) interleukin (IL)-22, and presents studies of the functional activity of its recombinant protein for the first time in a non-mammalian species. The predicted IL-22 coding region consists of 522 nucleotides which translates into a 173 amino acid protein, that contains an IL-10 family signature which is reasonably well conserved with other vertebrate IL-22 molecules. Expression analysis in tissues from healthy fish revealed a higher constitutive expression of IL-22 in mucosal tissues, suggesting a potentially important role in mucosal immunity. In vitro studies demonstrated that IL-22 expression was induced significantly by PHA and PMA in splenocyte primary cultures 4 h post-stimulation. Expression was also induced in the spleen upon infection of fish with the Gram-negative bacterium Yersinia ruckeri, suggesting a potential role of IL-22 in vivo in defence against bacterial diseases. The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart.
► This report describes the cloning and characterisation of rainbow trout interleukin (IL)-22.
► Expression analysis revealed a higher constitutive expression of IL-22 in mucosal tissues.
► PHA and PMA were good inducers of trout IL-22 expression in vitro.
► IL-22 expression was induced in the spleen of fish infected with the bacterium Yersinia ruckeri.
► E.coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides.
Journal: Cytokine - Volume 55, Issue 1, July 2011, Pages 62–73