کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2795546 1155331 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cigarette smoke extract induced cytokine and chemokine gene expression changes in COPD macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Cigarette smoke extract induced cytokine and chemokine gene expression changes in COPD macrophages
چکیده انگلیسی

Macrophages are key inflammatory cells in chronic obstructive pulmonary disease (COPD). The transcriptional regulation of inflammatory signalling pathways by cigarette smoke (CS) in COPD macrophages is not well understood. We have studied the effects of acute CS exposure on COPD macrophage cytokine, chemokine and signal transduction gene expression profiles. Monocyte derived macrophages (MDMs) from whole blood from patients with COPD (n = 6) were stimulated with 1%, 10% and 25% CS extract (CSE) for 6 h for microarray and quantitative polymerase chain reaction (Q-PCR) analysis. We observed a CSE dose dependant increase in the numbers of significantly regulated genes; 24, 340 and 627 genes at 1%, 10% and 25% CSE, respectively. IL-8 mRNA levels were up-regulated by 10% CSE (2.25-fold increase, 95% CI 1.28–4.00). In contrast a range of other cytokines and chemokines were down-regulated at both 10% and 25% CSE, including IL-1β, -6, -10 and -18, chemokine ligands CCL-2, -3, -4, -5, -8, -15, -20 and CXCL-1, -2 and -10. Q-PCR and microarray data were highly correlated (r = 0.95, p = 

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 42, Issue 2, May 2008, Pages 205–216
نویسندگان
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