کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2795936 1155351 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
C/EBPβ serine 64, a phosphoacceptor site, has a critical role in LPS-induced IL-6 and MCP-1 transcription
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
C/EBPβ serine 64, a phosphoacceptor site, has a critical role in LPS-induced IL-6 and MCP-1 transcription
چکیده انگلیسی

C/EBPβ is a member of the CCAAT/enhancer binding protein family of transcription factors and has been shown to be a critical transcriptional regulator of various proinflammatory genes, including IL-6 and MCP-1. Serine 64 in the transactivation domain of C/EBPβ has recently been identified as a Ras-induced phosphoacceptor site. The integrity of serine 64 along with threonine 189 is important for the Ha-rasV12-induced transformation of NIH3T3 cells, however no target genes dependent upon serine 64 for their expression have been reported. In order to evaluate a potential role of serine 64 in C/EBPβ-regulated cytokine expression, we expressed a form of C/EBPβ with an alanine substitution at serine 64 (C/EBPβS64A) in P388 murine B lymphoblasts, which lack endogenous C/EBPβ expression and are normally unresponsive to LPS for expression of IL-6 and MCP-1. In comparison to wild type C/EBPβ, which robustly supports the LPS-induced expression of IL-6 and MCP-1, C/EBPβS64A was severely impaired in its ability to support the LPS-induced transcription of IL-6 and MCP-1. Furthermore, LPS stimulation increased the level of phosphorylation detected at serine 64. Thus, serine 64, probably through its phosphorylation, is a critical determinant of C/EBPβ activity in the transcription of IL-6 and MCP-1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 37, Issue 2, February 2007, Pages 119–127
نویسندگان
, , , , , , ,