کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2795998 1568744 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Salbutamol inhibits trypsin-mediated production of CXCL8 by keratinocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Salbutamol inhibits trypsin-mediated production of CXCL8 by keratinocytes
چکیده انگلیسی
Treatment of primary keratinocytes (HEKAp) with trypsin led to the production and release of CXCL8. Production of CXCL8 was exquisitely sensitive to inhibition by co-treatment with the β2 agonist sabutamol (IC50 = 1.1 nM). The inhibitory effect of salbutamol was β receptor-mediated since the effect was prevented by the β antagonist sotalol. Salbutamol also elevated intracellular levels of cAMP (EC50 = 82 nM) but the relationship to the inhibition of CXCL8 secretion was not clear-cut since much higher concentrations of salbutamol were required to elevate total cellular cAMP than inhibit CXCL8 production. However, the effect of salbutamol is likely to be mediated by elevation of cAMP since forskolin, an adenylyl cyclase activator, mimicked the effects of salbutamol while the adenylyl cyclase inhibitor 2′,5′-dideoxyadenosine inhibited the effects of salbutamol. Potentiation of cAMP production by co-treatment with the phosphodiesterase type 4 inhibitor rolipram only marginally enhanced the inhibitory effect of salbutamol on CXCL8 production. Taken together, these data suggest that elevation of cAMP production is required for the inhibitory effect of salbutamol on CXCL8 production by keratinocytes and that low threshold levels of cAMP are sufficient to mediate this effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 36, Issues 1–2, October 2006, Pages 29-34
نویسندگان
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