کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2799841 1568877 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Different intracellular signalling properties induced by human and porcine growth hormone
ترجمه فارسی عنوان
خواص سیگنالینگ مختلف داخل سلولی ناشی از هورمون رشد انسان و سوسیس
کلمات کلیدی
هورمون رشد انسان، هورمون رشد گوشت خوک، فعالیت بیولوژیکی، سیگنالینگ داخل سلولی، نخستین غیر اولیه
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
چکیده انگلیسی


• The biological activities of primate GH and non-primate GH are different.
• We explored the issue from the angle of GH-induced intracellular signaling.
• We observed the main signalling proteins phosphorylation triggered by hGH or pGH.
• The mechanism explains the different biological activities between hGH and pGH.

Growth hormone (GH) is reportedly species-specific. Primate growth hormone can trigger non-primate growth hormone receptor (GHR), but primates GHR cannot be activated by non-primate GH. However, it is also unclear that why primate GH and non-primate GH have different biological activities. Thus, we analysed primate growth hormone (human growth hormone (hGH)) or non-primate GH (porcine growth hormone (pGH))-induced intracellular signalling in 3T3-F442A cells and rat hepatocytes in a dose- and time-dependent manner to explore the different biological activities between them. The results revealed that both hGH and pGH can activate Janus kinase 2 (JAK2), Signal transducers and activators of transcription 1, 3 and 5 (STATs 1, 3 and 5) and extracellular signal-regulated kinase 1/2 (ERK1/2). There were no significant differences in JAK2 or ERK1/2 tyrosine phosphorylation after hGH and pGH treatment, but there were different between hGH and pGH in STAT/1/3/5 tyrosine phosphorylation, and JAK2, STAT/1/3/5 tyrosine phosphorylation was time-dependent and dose-dependent, whereas ERK1/2 was not. Both hGH and pGH demonstrated similar kinetics for STATs 1, 3 and 5 phosphorylation, but the pGH-mediated tyrosine phosphorylation was weaker than that mediated by hGH. Our observations indicated that the levels of main signalling proteins phosphorylation triggered by hGH or pGH were not exactly the same, which may explain the different biological activities showed by primate GH and non-primate GH.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 229, 1 April 2016, Pages 67–73
نویسندگان
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