کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2800038 1568893 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of long-term corticosterone treatment on blood cell differentials and function in laboratory and wild-caught amphibian models
ترجمه فارسی عنوان
تأثیر درمان طولانی مدت کورتیکواسترولون بر اختلافات و عملکرد سلول های خونی در مدل های دوزیستان آزمایشگاهی و غواصی
کلمات کلیدی
کورت، کورتیکواسترون کورتیکواسترون، فشار، دوزیستان، لکوسیت، پشت سر گذاشتن اکسیداتیو
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
چکیده انگلیسی


• The effect of long-term stress on amphibian immunity is not well understood.
• We elevated plasma corticosterone in Xenopus laevis and Lithobates catesbeianus.
• Long-term elevated corticosterone altered blood cell differentials and function.
• Stressful habitat conditions may alter amphibian immunity for long-term periods.

The effect of long-term stress on amphibian immunity is not well understood. We modeled a long-term endocrine stress scenario by elevating plasma corticosterone in two species of amphibians and examined effects on white blood cell differentials and innate immune activity. Plasma corticosterone was elevated in American bullfrogs (Lithobates catesbeianus) by surgically implanting corticosterone capsules and in African clawed frogs (Xenopus laevis) by immersion in corticosterone-treated water. To provide a context for our results within endogenous corticosterone fluctuations, diurnal plasma corticosterone cycles were determined. A daily low of corticosterone was observed in X. laevis at 12:00, while a significant pattern was not observed in L. catesbeianus. Elevated plasma corticosterone levels increased the ratio of peripheral neutrophils to lymphocytes, in both species, and decreased eosinophil concentrations in L. catesbeianus over a long-term period. Whole blood oxidative burst generally correlated with neutrophil concentrations, and thus was increased with corticosterone treatment, significantly in L. catesbeianus. In L. catesbeianus, an endogenous response of eosinophils and lymphocytes to implanted empty (sham) capsules was observed, but this effect was attenuated by corticosterone. Peripheral monocyte and basophil concentrations were not significantly altered by corticosterone treatment in either species. Our results show that long-term stress can alter amphibian immune parameters for extended periods and may play a role in susceptibility to disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 212, 1 February 2015, Pages 73–83
نویسندگان
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