Sex steroid-independent effects of photostimulation on the song-control system of white-throated sparrows (Zonotrichia albicollis)

• Songbirds were castrated and treated with flutamide and ATD.
• Growth of HVC and RA independent of sex steroids was observed.
• Photostimulation did not affect DCX-immunoreactivity in HVC.
• We report sex steroid-independent effects of photostimulation on song system growth.

Brain nuclei within the song-control system of songbirds are seasonally plastic during adulthood. These nuclei are larger in birds exposed to long, spring-like days than short, winter-like days. There is overwhelming evidence that this effect is mediated by testosterone (T). However, castration studies have also demonstrated that photostimulation has gonad-independent effects on song-control system plasticity, but these studies rarely control for extra-gonadal sources of T. In this study, we used anti-androgen and anti-estrogen treatments in combination with castration to determine the sex steroid-independent effects of photostimulation on HVC size and doublecortin immunoreactivity in white-throated sparrows (Zonotrichia albicollis). Birds were kept on short days or photostimulated for 1 month. Photostimulated birds were intact, castrated and treated with anti-androgens and anti-estrogens, or castrated and treated with T. HVC volumes of photostimulated birds were significantly larger than short-day birds. HVC volume of castrated birds given anti-androgens/-estrogens was significantly larger than short-day birds, indicating a sex steroid-independent effect of photostimulation. Similar results were observed for RA. The number of migrating neurons (immunoreactive for doublecortin) in HVC did not differ between treatment groups. Our data support the view that photostimulation alone can drive song-control system nuclei growth, and that concurrent exposure to T potentiates this growth. Moreover, these effects do not appear dependent on modulation of neuron migration.