Circulating leptin levels do not reflect the amount of body fat in the dunlin Calidris alpina during migration

• We examined if circulating leptin levels correlate with body fat in wild birds.
• Plasma leptin levels were determined in 150 adult dunlins during autumn migration.
• Birds were divided into three groups according to fat score.
• In dunlins, plasma leptin levels do not increase with adiposity.
• Leptin does not signal body fat reserves nor energy status in birds during migration.

Leptin is a peptide hormone that plays an important role in the regulation of energy homeostasis. Studies in mammals have shown that circulating leptin levels reflect adiposity and that this adipocyte-derived cytokine acts as an afferent satiety signal to the brain, decreasing food intake and increasing energy expenditure. Since leptin has been found in the liver and adipose tissue of migratory birds that are able to accumulate fat reserves as endogenous fuel for flight, we hypothesized that individuals with higher fat score would have higher plasma leptin levels, as it had been found previously in mammals. The aim of this study was to determine if circulating leptin levels correlate with the amount of body fat in a migratory bird, the dunlin Calidris alpina. Adult dunlins were caught during autumn migration on the Baltic coast, and their fat score was determined. Blood samples from 150 birds were used to assess the levels of circulating leptin. We did not find any statistical differences between dunlins with various fat scores. In fact, plasma leptin levels tended to be lower in fat birds than in lean individuals. Our data indicate that in wild birds in migration mode leptin does not reflect the amount of accumulated fat. It suggests that leptin in birds during migration is neither involved in the regulation of energy homeostasis nor acts as a signal to control the amount of body fat.