Effect of oxytocin, prolactin-releasing peptide, or corticotropin-releasing hormone on feeding behavior in steers

As a preliminary step to elucidate the involvement of central neurotransmitters in the dip in voluntary feed intake during the perinatal period in cows, we investigated the effect of intracerebroventricular (ICV) administration of oxytocin, prolactin-releasing peptide (PrRP), or corticotropin-releasing hormone (CRH), the central functions of all of which undergo drastic changes during the perinatal period, on feed intake in steers. Thirty minutes before the onset of feeding, the treatment solution was injected into the third ventricle through an implanted cannula, and feeding-related behaviors were observed for 1 h after the onset of feeding. Neither ICV oxytocin (5 and 50 μg) nor PrRP (2 and 20 nmol) reduced feed intake (n = 6). Twenty nanomoles of bovine CRH noticeably inhibited feeding behavior compared with vehicle treatment (n = 5, p < 0.05). Fifty micrograms of oxytocin reduced latency to the first water access after feeding onset (p < 0.1), which may be because of the stimulation of arginine vasopressin V1b receptor by the high dose of oxytocin.We conclude that CRH inhibits feeding behavior by its central action in this species, although this could also be an indirect effect due to the increased expression of abnormal behaviors caused by CRH. Central administration of neither oxytocin nor PrRP reduced feed intake in steers. Although the effects of sex steroids need to be examined, it appears that increased activity of oxytocin, and possibly PrRP, during the perinatal period does not contribute to the dip in voluntary feed intake in this species. On the other hand, it makes sense that suppressed central CRH activity during the perinatal period should act in the direction of maintaining or even increasing food intake to aid a steady supply of energy to the fetus or offspring. We thus speculate that CRH is not a prime candidate involved in the dip in voluntary feed intake during the perinatal period in cows.

► We investigated candidates for central satiating signal in steer.
► Bovine corticotropin-releasing hormone (CRH) inhibited feeding behavior.
► Neither centrally administered oxytocin nor PrRP reduced food intake.
► CRH could be a central satiety signal in cattle like other species.
► Species difference from rodents in the role of central oxytocin and PrRP exist.