کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2801223 1156148 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of RU486 on cyclooxygenase-2 gene expression, prostaglandin F2α synthesis and ovulation in Xenopus laevis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Effects of RU486 on cyclooxygenase-2 gene expression, prostaglandin F2α synthesis and ovulation in Xenopus laevis
چکیده انگلیسی

RU486 is a synthetic analog of progesterone and functions as a progesterone receptor antagonist. It binds to the progesterone receptor to prevent progesterone from occupying its receptor in many cellular systems. Early studies from our laboratory have shown that in Xenopus laevis ovarian follicles progesterone stimulates the expression of cyclooxygenase-2 (COX-2) gene which leads to a rapid increase in the production of prostaglandin F2α (PGF2α) and subsequent ovulation. In this study, we examined the effect of RU486 on the synthesis of COX-2 mRNA, production of PGF2α and ovulation in X. laevis. Ovarian tissue fragments were primed with human chorionic gonadotropin (hCG) and then incubated with progesterone (P4) alone or in the presence of varying concentrations of RU486 over a period of 12 h. After the incubation ovulated oocytes were counted, COX-2 expression and synthesis of PGF2α were measured. Results demonstrated that RU486 attenuated the expression of COX-2 gene, reduced the synthesis of PGF2α, and inhibited ovulation in a dose-dependent manner. This finding suggests that progesterone receptor is an important regulator in the progesterone–cyclooxygenase–prostaglandin-mediated ovulation in amphibians.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 165, Issue 1, 1 January 2010, Pages 78–82
نویسندگان
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