کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2801909 1156180 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a luteinizing hormone-selective determinant in the exodomain of a follicle-stimulating hormone receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Identification of a luteinizing hormone-selective determinant in the exodomain of a follicle-stimulating hormone receptor
چکیده انگلیسی

Mammalian glycoprotein hormone receptors (GpHRs) display a stringent selectivity for their cognate hormones. In contrast, the follicle-stimulating hormone receptor of the African catfish (cfFSHR) is promiscuously activated by catfish luteinizing hormone (cfLH). Glycoprotein hormones bind to the concave site of the cusp-shaped N-terminal GpHR exodomain, which is formed by 9–10 parallel β-strands. Hence, hormone selectivity of each GpHR for its cognate ligand is defined by amino acid sequence divergence in these β-strands between different GpHRs. To identify the molecular determinants that allow promiscuous activation of the cfFSHR by cfLH, β-strands were systematically exchanged between the cfFSHR and the human FSHR. Both gain-of-function and loss-of-function mutational approaches revealed that β-strand 2 of the cfFSHR contains determinants that contribute to the receptor’s responsiveness to cfLH.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 156, Issue 3, 1 May 2008, Pages 490–498
نویسندگان
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