کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2802141 | 1156188 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cortisol is necessary for seawater tolerance in larvae of a marine teleost the summer flounder
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
Larval-stage summer flounder (Paralichthys dentatus) were immersed in the corticosteroid-receptor blocker RU486 to test the effects of cortisol deficiency on salinity tolerance. Premetamorphic larvae held at 10 (near isosmotic) or 30 (hyperosmotic) parts per thousand (â°) seawater survived well over 5 d in 0, 0.012, or 0.12 μM RU486. However, at concentrations of 1.2 or 3.6 μM RU486, mortality was significantly greater for larvae in 30â° compared to larvae in 10â°. In a separate experiment, the ability of RU486 to inhibit tolerance to hyperosmotic medium (30â°) was confirmed; immersion at 1.2 μM RU486 induced mortality of larvae in the metamorphic climax stage held at 30â°, but not 0 or 10â°. Mortality due to RU486 in pre- or prometamorphic stage larvae was prevented by concurrent immersion in cortisol at concentrations approximately 10-200 times greater than RU486, indicating that the action of RU486 was specific to antagonism of cortisol. The efficacy of 1.2 μM RU486 in reducing survival in 30â° was found to be stage-dependent and exhibited the following hierarchy for fastest time to 50% mortality: prometamorphosis > metamorphic climax > premetamorphosis. In a 5-d pretreatment of pre- or prometamorphic larvae by immersion in 20 μM cortisol and/or 0.12 μM RU486 at 30â°, only RU486 had a limited effect on decreasing survival when larvae were challenged with abrupt exposure to 50â°. In total, the results evidence for the first time a necessary role for cortisol in seawater tolerance of a larval marine teleost.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 151, Issue 1, March 2007, Pages 116-121
Journal: General and Comparative Endocrinology - Volume 151, Issue 1, March 2007, Pages 116-121
نویسندگان
Philip A. Veillette, Maricruz Merino, Neil D. Marcaccio, Misty M. Garcia, Jennifer L. Specker,