Complete genome sequencing and comparative analyses of broad-spectrum antimicrobial-producing Micromonospora sp. HK10

• Micromonospora sp. HK10, a soil isolate with broad-spectrum antimicrobial activity against various human pathogens.
• HK10 genome has been sequenced using Illumina NexSeq 500 platform and de novo assembled.
• HK10 genome consists of 6,911,179 bp with 73.39% GC content and contains 6,196 protein coding genes.
• Total 18 Genetic Islands and 49 secondary metabolite clusters (13-PKS, 2-NRPS and 34-other) has been predicted.
• Seven unique secondary metabolite clusters (3 horizontally transferred) has been identified in HK10 genome.

Micromonospora genus produces > 700 bioactive compounds of medical relevance. In spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing Micromonospora strains. Thus, here we contribute the full genome sequence of Micromonospora sp. HK10 strain, which has high antibacterial activity against several important human pathogens like, Mycobacterium abscessus, Mycobacterium smegmatis, Bacillus subtillis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella and Escherichia coli. We have generated whole genome sequence data of Micromonospora sp. HK10 strain using Illumina NexSeq 500 sequencing platform (2 × 150 bp paired end library) and assembled it de novo. The sequencing of HK10 genome enables identification of various genetic clusters associated with known- and probably unknown- antimicrobial compounds, which can pave the way for new antimicrobial scaffolds.

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